Identification and characterization of Japanese encephalitis virus envelope protein gene from swine
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چکیده
منابع مشابه
Identification and characterization of the RNA helicase activity of Japanese encephalitis virus NS3 protein.
The NS3 protein of Japanese encephalitis virus (JEV) contains motifs typical of RNA helicase/NTPase but no RNA helicase activity has been reported for this protein. To identify and characterize the RNA helicase activity of JEV NS3, a truncated form of the protein with a His-tag was expressed in Escherichia coli and purified. The purified JEV NS3 protein showed an RNA helicase activity, which wa...
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Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97....
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The role of the Japanese encephalitis virus (JEV) premembrane (prM) protein in maturation of the envelope (E) glycoprotein was evaluated by using recombinant vaccinia viruses encoding E in the presence (vP829) or absence (vP658) of prM. Immunofluorescence analyses showed that E appeared to be localized in the endoplasmic reticulum of cells infected with JEV, vP829, or vP658. However, reactivity...
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Several mouse central nervous system genes have been identified that are differentially regulated during Japanese encephalitis virus (JEV) infection, including those which have not been reported to be induced by any other neurotropic virus. Interestingly, approximately 80 % of JEV-inducible genes identified in this study are also induced by Sindbis virus, indicating activation of common host si...
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*Corresponding author: Mailing address: Tama Branch Institute, Tokyo Metropolitan Institute of Public Health, 3-16-25 Shibasakicho, Tachikawa, Tokyo 190-0023, Japan. Tel: +81-42-524-8263, Fax: +81-42-524-5307, E-mail: [email protected]. tokyo.jp Japanese encephalitis (JE) is a serious viral encephalitis caused by a mosquito-borne flavivirus, the Japanese encephalitis virus (JEV). In t...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2010
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2010.02850.x